A polypeptide with sequence of qkvdssggggs was designed to be a linker between c terminal of penicillin g acylase and n terminal of the coat protein . the ribosome binding site ( rbs sequence ) of psurfscript is also replaced by rbs sequence originating from bacillus subtilis . it was demonstrated that constructed phagemid can still express penicillin g acylase 将包含信号肽和琥珀终止密码子uag ( amber )的完整巨大芽孢杆菌青霉素g酰化酶基因克隆到噬菌粒psurfscript ，通过引入的11肽连接青霉素g酰化酶的c末端与噬菌体外壳蛋白gp3的n末端。
